In silico prediction of Ara h 2 T cell epitopes in peanut‐allergic children
Identifieur interne : 002135 ( Main/Exploration ); précédent : 002134; suivant : 002136In silico prediction of Ara h 2 T cell epitopes in peanut‐allergic children
Auteurs : M. Pascal [États-Unis, Espagne] ; G. N. Konstantinou [États-Unis, Grèce] ; M. Masilamani [États-Unis] ; J. Lieberman [États-Unis] ; H. A. Sampson [États-Unis]Source :
- Clinical & Experimental Allergy [ 0954-7894 ] ; 2013-01.
Abstract
Background: Despite the frequency and severity of peanut allergy, the only approved treatment is strict avoidance. Different types of immunotherapy with crude peanut extract are not universally effective and have been associated with relatively high adverse reaction rates. Objective: We sought to determine whether in silico predictive algorithms were useful in identifying candidate peptides for an Ara h 2 peptide‐based vaccine using peanut‐allergic patients' peripheral blood mononuclear cells (PBMCs) in vitro. A human leucocyte antigen (HLA) distribution analysis was also performed. Methods: Major histocompatibility complex (MHC)‐class II‐binding peptides were predicted using NetMHCIIpan‐2.0 and NetMHCII‐2.2 algorithms. PBMCs from 80 peanut‐allergic patients were stimulated with overlapping 20‐mer Ara h 2 peptides. Cell supernatant cytokine profiles were evaluated by multiplex assays. HLA‐DRB1* and HLA‐DQB1* typing were performed. Results: Four regions of overlapping sequences induced PBMC proliferation and predominant Th2 cytokine production. HLA genotyping showed 30 different DRB1* allele specificities and eight DQ serological specificities. The in silico analysis revealed similar relevant regions and predicted identical or similar core 9‐mer epitopes to those identified in vitro. If relevant peptides, as determined by either in vitro or in silico analysis (15 peptides and 9 core epitopes respectively), were used in a peptide‐based vaccine, they would cover virtually all subjects in the cohort studied. Conclusions and Clinical Relevance: Four dominant regions in Ara h 2 have been identified, containing sequences that could serve as potential candidates for peptide‐based immunotherapy. MHC‐class II‐based T cell epitope prediction algorithms for HLA‐DR and ‐DQ loci accurately predicted Ara h 2 T cell epitopes in peanut‐allergic subjects, suggesting their potential utility in a peptide‐based vaccine design for food allergy.
Url:
DOI: 10.1111/cea.12014
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000185
- to stream Istex, to step Curation: 000185
- to stream Istex, to step Checkpoint: 000273
- to stream Main, to step Merge: 002159
- to stream Main, to step Curation: 002135
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">In silico prediction of Ara h 2 T cell epitopes in peanut‐allergic children</title>
<author><name sortKey="Pascal, M" sort="Pascal, M" uniqKey="Pascal M" first="M." last="Pascal">M. Pascal</name>
</author>
<author><name sortKey="Konstantinou, G N" sort="Konstantinou, G N" uniqKey="Konstantinou G" first="G. N." last="Konstantinou">G. N. Konstantinou</name>
</author>
<author><name sortKey="Masilamani, M" sort="Masilamani, M" uniqKey="Masilamani M" first="M." last="Masilamani">M. Masilamani</name>
</author>
<author><name sortKey="Lieberman, J" sort="Lieberman, J" uniqKey="Lieberman J" first="J." last="Lieberman">J. Lieberman</name>
</author>
<author><name sortKey="Sampson, H A" sort="Sampson, H A" uniqKey="Sampson H" first="H. A." last="Sampson">H. A. Sampson</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:842662877DFE278C5B8157CC24A293056EC19EF5</idno>
<date when="2013" year="2013">2013</date>
<idno type="doi">10.1111/cea.12014</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-21VMVR19-W/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000185</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000185</idno>
<idno type="wicri:Area/Istex/Curation">000185</idno>
<idno type="wicri:Area/Istex/Checkpoint">000273</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000273</idno>
<idno type="wicri:doubleKey">0954-7894:2013:Pascal M:in:silico:prediction</idno>
<idno type="wicri:Area/Main/Merge">002159</idno>
<idno type="wicri:Area/Main/Curation">002135</idno>
<idno type="wicri:Area/Main/Exploration">002135</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">In silico prediction of Ara h 2 T cell epitopes in peanut‐allergic children</title>
<author><name sortKey="Pascal, M" sort="Pascal, M" uniqKey="Pascal M" first="M." last="Pascal">M. Pascal</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Allergy & Immunology and The Jaffe Food Allergy Research Institute, Mount Sinai School of Medicine, New York, NY</wicri:regionArea>
<wicri:noRegion>NY</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Immunology, Allergy Unit, Centre de Diagnòstic Biomèdic (CDB), Hospital Clínic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona</wicri:regionArea>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Konstantinou, G N" sort="Konstantinou, G N" uniqKey="Konstantinou G" first="G. N." last="Konstantinou">G. N. Konstantinou</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Allergy & Immunology and The Jaffe Food Allergy Research Institute, Mount Sinai School of Medicine, New York, NY</wicri:regionArea>
<wicri:noRegion>NY</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Grèce</country>
<wicri:regionArea>Department of Allergy, 424 General Military Training Hospital, Thessaloniki</wicri:regionArea>
<wicri:noRegion>Thessaloniki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Masilamani, M" sort="Masilamani, M" uniqKey="Masilamani M" first="M." last="Masilamani">M. Masilamani</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Allergy & Immunology and The Jaffe Food Allergy Research Institute, Mount Sinai School of Medicine, New York, NY</wicri:regionArea>
<wicri:noRegion>NY</wicri:noRegion>
</affiliation>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Immunology Institute, Mount Sinai School of Medicine, NY, New York</wicri:regionArea>
<placeName><region type="state">État de New York</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lieberman, J" sort="Lieberman, J" uniqKey="Lieberman J" first="J." last="Lieberman">J. Lieberman</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Allergy & Immunology and The Jaffe Food Allergy Research Institute, Mount Sinai School of Medicine, NY, New York</wicri:regionArea>
<placeName><region type="state">État de New York</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sampson, H A" sort="Sampson, H A" uniqKey="Sampson H" first="H. A." last="Sampson">H. A. Sampson</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Allergy & Immunology and The Jaffe Food Allergy Research Institute, Mount Sinai School of Medicine, New York, NY</wicri:regionArea>
<wicri:noRegion>NY</wicri:noRegion>
</affiliation>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Immunology Institute, Mount Sinai School of Medicine, NY, New York</wicri:regionArea>
<placeName><region type="state">État de New York</region>
</placeName>
</affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Clinical & Experimental Allergy</title>
<title level="j" type="alt">CLINICAL AND EXPERIMENTAL ALLERGY</title>
<idno type="ISSN">0954-7894</idno>
<idno type="eISSN">1365-2222</idno>
<imprint><biblScope unit="vol">43</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="116">116</biblScope>
<biblScope unit="page" to="127">127</biblScope>
<biblScope unit="page-count">12</biblScope>
<date type="published" when="2013-01">2013-01</date>
</imprint>
<idno type="ISSN">0954-7894</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0954-7894</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Background: Despite the frequency and severity of peanut allergy, the only approved treatment is strict avoidance. Different types of immunotherapy with crude peanut extract are not universally effective and have been associated with relatively high adverse reaction rates. Objective: We sought to determine whether in silico predictive algorithms were useful in identifying candidate peptides for an Ara h 2 peptide‐based vaccine using peanut‐allergic patients' peripheral blood mononuclear cells (PBMCs) in vitro. A human leucocyte antigen (HLA) distribution analysis was also performed. Methods: Major histocompatibility complex (MHC)‐class II‐binding peptides were predicted using NetMHCIIpan‐2.0 and NetMHCII‐2.2 algorithms. PBMCs from 80 peanut‐allergic patients were stimulated with overlapping 20‐mer Ara h 2 peptides. Cell supernatant cytokine profiles were evaluated by multiplex assays. HLA‐DRB1* and HLA‐DQB1* typing were performed. Results: Four regions of overlapping sequences induced PBMC proliferation and predominant Th2 cytokine production. HLA genotyping showed 30 different DRB1* allele specificities and eight DQ serological specificities. The in silico analysis revealed similar relevant regions and predicted identical or similar core 9‐mer epitopes to those identified in vitro. If relevant peptides, as determined by either in vitro or in silico analysis (15 peptides and 9 core epitopes respectively), were used in a peptide‐based vaccine, they would cover virtually all subjects in the cohort studied. Conclusions and Clinical Relevance: Four dominant regions in Ara h 2 have been identified, containing sequences that could serve as potential candidates for peptide‐based immunotherapy. MHC‐class II‐based T cell epitope prediction algorithms for HLA‐DR and ‐DQ loci accurately predicted Ara h 2 T cell epitopes in peanut‐allergic subjects, suggesting their potential utility in a peptide‐based vaccine design for food allergy.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>Grèce</li>
<li>États-Unis</li>
</country>
<region><li>Catalogne</li>
<li>État de New York</li>
</region>
<settlement><li>Barcelone</li>
</settlement>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Pascal, M" sort="Pascal, M" uniqKey="Pascal M" first="M." last="Pascal">M. Pascal</name>
</noRegion>
<name sortKey="Konstantinou, G N" sort="Konstantinou, G N" uniqKey="Konstantinou G" first="G. N." last="Konstantinou">G. N. Konstantinou</name>
<name sortKey="Lieberman, J" sort="Lieberman, J" uniqKey="Lieberman J" first="J." last="Lieberman">J. Lieberman</name>
<name sortKey="Masilamani, M" sort="Masilamani, M" uniqKey="Masilamani M" first="M." last="Masilamani">M. Masilamani</name>
<name sortKey="Masilamani, M" sort="Masilamani, M" uniqKey="Masilamani M" first="M." last="Masilamani">M. Masilamani</name>
<name sortKey="Sampson, H A" sort="Sampson, H A" uniqKey="Sampson H" first="H. A." last="Sampson">H. A. Sampson</name>
<name sortKey="Sampson, H A" sort="Sampson, H A" uniqKey="Sampson H" first="H. A." last="Sampson">H. A. Sampson</name>
<name sortKey="Sampson, H A" sort="Sampson, H A" uniqKey="Sampson H" first="H. A." last="Sampson">H. A. Sampson</name>
</country>
<country name="Espagne"><region name="Catalogne"><name sortKey="Pascal, M" sort="Pascal, M" uniqKey="Pascal M" first="M." last="Pascal">M. Pascal</name>
</region>
</country>
<country name="Grèce"><noRegion><name sortKey="Konstantinou, G N" sort="Konstantinou, G N" uniqKey="Konstantinou G" first="G. N." last="Konstantinou">G. N. Konstantinou</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002135 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002135 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:842662877DFE278C5B8157CC24A293056EC19EF5 |texte= In silico prediction of Ara h 2 T cell epitopes in peanut‐allergic children }}
This area was generated with Dilib version V0.6.33. |